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Non-isothermal cephalexin hydrolysis by penicillin G acylase immobilized on grafted nylon membranes |
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| Authors: | M S Mohy Eldin M Santucci S Rossi U Bencivenga P Canciglia F S Gaeta J Tramper A E M Janssen C G P H Schroen D G Mita |
| Institution: | a International Institute of Genetics and Biophysics, CNR Via Marconi, 12, 80125 Naples, Italy b Department of Human Physiology and Integrated Biological Functions, II University of Naples, Via S.M. di Costantinopoli, 16, 80138 Naples, Italy c Department of Food Technology and Nutritional Sciences, Wageningen Agricultural University, P.O. Box 8129, 6700 EV Wageningen, Netherlands |
| Abstract: | A new catalytic membrane has been prepared using a nylon membrane grafted by γ-radiation with methylmethacrylate (MMA) and using hexamethylenediamine (HMDA) as spacer. Penicillin G acylase (PGA) and cephalexin were employed as catalyst and substrate, respectively. Cephalexin hydrolysis was studied in bioreactors operated under isothermal and non-isothermal conditions. A hydrolysis increase was found when the temperature of the warm membrane surface was kept constant and the temperature of the other membrane surface was kept at a lower value. The hydrolysis increase was linearly proportional to the applied temperature difference. Cephalexin hydrolysis increased to about 10% when a temperature difference of 1°C was applied across the catalytic membrane. These results have been attributed to the non-isothermal cephalexin transport across the membrane, i.e., to the process of thermodialysis. In this way, the enzyme immobilized on and into the membrane reacts with a substrate concentration higher than that produced by simple diffusion under isothermal conditions. |
| Keywords: | Immobilized enzymes Biocatalytic membranes Penicillin G acylase γ-Radiation Non-isothermal bioreactors Cephalexin |
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