Low infectivity of vesicular stomatitis virus (VSV) particles released from interferon-treated cells is related to glycoprotein deficiency |
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Authors: | R K Maheshwari M M Husain R M Friedman |
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Affiliation: | School of Chemistry, Georgia Institute of Technology, Atlanta, Ga. 30332 USA |
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Abstract: | We have investigated the mechanism for the low infectivity of vesicular stomatitis virus (VSV) released from interferon (IFN) -treated cells. With 10-30 units/ml of IFN there was an approximately 5-30 fold reduction in the production of virus particles, as measured by VSV proteins; however, the infectivity of the VSV released from IFN-treated mouse LB, JLS-V9R, or human GM2504 was drastically reduced (2 to 4 logs). The low infectivity of VSV was directly related to a deficiency in virion glycoprotein (G). IFN treatment did not change the specific infectivity of the VSV particles released by HeLa cells; their G protein was also not reduced. A further effect of IFN to reduce the amount of virion M protein appeared to be secondary and was probably not related to the reduced infectivity of VSV. |
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Keywords: | DBH dopamine-B-hydroxylase HPLC high performance liquid chromatography SNPA N-succinimidyl-p-nitrophenylacetate PAME 1-phenyl-1-aminomethylethene DHPPA 2,3-dihydroxy-2-phenyl-1-propylamine MES 2-(N-morpholino)-ethanesulfonic acid |
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