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Suppression of type I collagen production by microRNA-29b in cultured human stellate cells |
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| Authors: | Tomohiro Ogawa Yumiko Sekiya Katsutoshi Yoshizato Kazuo Ikeda |
| Institution: | a Department of Hepatology, Graduate School of Medicine, Osaka City University, Osaka, Japan b Toray Industries Inc., Kanagawa, Japan c PhoenixBio Co. Ltd., Hiroshima, Japan d Department of Functional Anatomy, Graduate School of Medicine, Nagoya City University, Aichi, Japan |
| Abstract: | MicroRNAs (miRNAs) are small noncoding RNAs that regulate gene expression through imperfect base pairing with the 3′ untranslated region (3′UTR) of target mRNA. We studied the regulation of alpha 1 (I) collagen (Col1A1) expression by miRNAs in human stellate cells, which are involved in liver fibrogenesis. Among miR-29b, -143, and -218, whose expressions were altered in response to transforming growth factor-β1 or interferon-α stimulation, miR-29b was the most effective suppressor of type I collagen at the mRNA and protein level via its direct binding to Col1A1 3′UTR. miR-29b also had an effect on SP1 expression. These results suggested that miR-29b is involved in the regulation of type I collagen expression by interferon-α in hepatic stellate cells. It is anticipated that miR-29b will be used for the regulation of stellate cell activation and lead to antifibrotic therapy. |
| Keywords: | Col1A1 alpha 1 (I) collagen DMEM Dulbecco&rsquo s modified Eagle&rsquo s medium FBS fetal bovine serum IFN interferon miRNAs microRNAs TGF-β transforming growth factor-β UTR untranslated region |
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