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Effect of cobalt and nickel on growth and carboxymethyl cellulase activity ofCellulomonas spp
Authors:Shailendra Saxena  J. Bahadur  Ajit Varma
Affiliation:(1) Microbiology Unit, School of Life Sciences, Jawaharlal Nehru University, 110067 New Delhi, India;(2) School of Studies in Zoology, Jiwaji University, 110067 Gwalior, India
Abstract:ACellulomonas spp isolated from soil produced carboxymethyl cellulase (CMCase) and xylanase enzymes. Cobalt (0.1mm) and nickel (0.1mm) decreased the growth rate ofCellulomonas spp. These metal ions activated CMCase activity but not xylanase activity; cobalt being the greater stimulatory ion than nickel. A predominant long lag phase was observed in adapted cells when compared with the non-adapted cells. However, the growth level of control cells was never obtained by the adapted cells.
Keywords:carboxymethyl cellulose  carboxymethyl cellulase  Cellulomonas spp, growth
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