Presence of Radiolabelled Metabolites in Release Studies Using [3H]γ-Aminobutyric Acid |
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Authors: | Colin R Gardner Mary H Richards |
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Institution: | Centre de Recherche Merrell International, Strashourg, France |
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Abstract: | Abstract: Most studies on γ-aminobutyric acid (GABA) release from nervous tissue have been conducted using radiolabelled GABA in the presence of aminooxyacetic acid (AOAA) to inhibit GABA: 2-oxoglutarate aminotransferase (GABA-T) to prevent conversion of labelled GABA to labeled catabolites. Here we present data showing that even in the presence of 10 μM-AOAA the spontaneous release of tritium from rat cortical synaptosomes prelabelled with 2,3-3H]GABA is mainly in the form of tritiated water but that the increase in tritium release in the presence of unlabelled GABA or high potassium-ion concentrations is in the form of authentic 3H]GABA. Interpretation of results should take these facts into account. |
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Keywords: | GABAR Release Synaptosomes Superfusion Metabolism GABA-transaminase |
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