The influence of mammalian and teleost somatostatins on the secretion of growth hormone from goldfish (Carassius auratus L.) pituitary fragments in vitro |
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| Authors: | T A Marchant R A Fraser P C Andrews R E Peter |
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| Institution: | 1. Department of Zoology, University of Alberta, Edmonton, Alta. Canada;2. Department of Biochemistry, Purdue University, West Lafayette, IN 47907 U.S.A.;1. Institute of Psychology II, Technische Universität Dresden, Dresden, Germany;2. MSH Medical School Hamburg, University of Applied Science and Medical University, Hamburg, Germany;3. Division of Molecular Psychiatry, Laboratory of Translational Neurobiology, Department of Psychiatry, Psychosomatics, and Psychotherapy, University of Wuerzburg, Wuerzburg, Germany;4. Institute of Psychology I, Technische Universität Dresden, Dresden, Germany;1. Department of Physiology and Biochemistry, Faculty of Veterinary Sciences, University of Nyala, Nyala, Sudan;2. Department of Microbiology, Faculty of Medical Laboratory Sciences, University of Khartoum, Khartoum, Sudan;3. Key Laboratory of Environmental Medicine, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, Jiangsu, China;4. Department of Biology, Faculty of Education, University of Nyala, Sudan;1. Pacific Salmon Ecology and Conservation Laboratory, Department of Forest and Conservation Sciences, University of British Columbia, Vancouver, BC, Canada;2. Department of Biology, University of Ottawa, Ottawa, ON, Canada;3. Fish Ecology and Conservation Physiology Laboratory, Department of Biology, Carleton University, 1125 Colonel By Drive, Ottawa, ON, Canada;4. Fisheries and Oceans Canada, Science Branch, Pacific Region, Cooperative Resource Management Institute, School of Resource and Environmental Management, Simon Fraser University, Burnaby, BC, Canada;1. Department of Biology and Medical Parasitology, Pomeranian Medical University, Powstanców Wielkopolskich 72, 70-111 Szczecin, Poland;2. Laboratory of Medical Rehabilitation, Pomeranian Medical University, Zolnierska 54, 71-210 Szczecin, Poland;3. Chair and Clinic of Orthopaedics, Traumatology and Oncology, Pomeranian Medical University, Unii Lubelskiej 1, 71-252 Szczecin, Poland;4. Department of Animal Hygiene and Prophylaxis, West Pomeranian University of Technology, Judyma 6, 71-466 Szczecin, Poland;5. Department of Ecology, Environmental Management and Protection, West Pomeranian University of Technology, Słowackiego 17, 71-434 Szczecin, Poland;1. Department of Pathology, Microbiology, and Immunology, School of Veterinary Medicine, University of California, Davis, CA, USA;2. Blood Systems Research Institute, 270 Masonic Avenue, San Francisco, CA, USA;3. Department of Laboratory Medicine, University of California, San Francisco, CA, USA |
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| Abstract: | The effect of various vertebrate somatostatins (SRIF) on basal growth hormone (GH) secretion from goldfish pituitary fragments was studied using an in vitro perifusion system. SRIF-14 caused a rapid and dose-dependent decrease in the rate of GH release from goldfish pituitary fragments. The half-maximal effective dose (ED50) of SRIF-14 was calculated as 1.3 nM following exposure to two minute pulses of increasing concentrations of SRIF-14, whereas the ED50 of SRIF-14 calculated after continuous exposure to sequentially increasing doses of SRIF-14 was 65 nM. This difference suggests that the pituitary fragments were less responsive to SRIF-14 in the latter experiment, possibly as a result of previous exposure to SRIF-14. SRIF-28 was found to be equipotent with SRIF-14 in decreasing basal GH secretion from the goldfish pituitary. In contrast, catfish SRIF-22, a uniquely teleost SRIF isolated from catfish pancreatic islets, did not alter GH secretion. These results provide further support for the hypothesis that SRIF-14 or a very similar molecule functions as a GH release-inhibiting factor in teleosts, indicating that this action of SRIF-14 has been fully conserved throughout vertebrate evolution. |
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