Adrenergic receptor characteristics of cardiac myocytes cultured in serum-free medium: comparison with serum-supplemented medium |
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| Authors: | J S Karliner P C Simpson J E Taylor N Honbo W Woloszyn |
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| Affiliation: | 1. Developmental Biochemistry, Theodor Boveri Institute (Biocenter), University of Wuerzburg, Am Hubland, D-97074 Wuerzburg, Germany;2. Physiological Chemistry, Theodor Boveri Institute (Biocenter), University of Wuerzburg, Am Hubland, D-97074 Wuerzburg, Germany;3. Biochemistry and Molecular Biology, Theodor Boveri Institute (Biocenter), University of Wuerzburg, Am Hubland, D-97074 Wuerzburg, Germany;4. Comprehensive Cancer Center Mainfranken, University of Wuerzburg, Am Hubland, D-97074 Wuerzburg, Germany;5. Comprehensive Heart Failure Center, University of Wuerzburg, Am Hubland, D-97074 Wuerzburg, Germany;1. Division of Cardiovascular Medicine, Department of Internal Medicine, Shuang Ho Hospital, Taipei Medical University, New Taipei City, Taiwan;2. Institute of Toxicology, College of Medicine, National Taiwan University, Taipei, Taiwan;3. Department of Radiation Oncology, Wan Fang Hospital, Taipei Medical University, Taipei, Taiwan;4. Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan;5. Department of Biotechnology, Hungkuang University, Taichung, Taiwan;1. Department of Structural and Functional Biology, Institute of Biology, University of Campinas — UNICAMP, Campinas, SP, Brazil;2. Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of São Paulo — USP, São Paulo, SP, Brazil |
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| Abstract: | Neonatal rat ventricular myocytes cultured in serum-free medium coexpress both alpha 1 and beta 1 receptors as determined by radioligand binding studies. In cells exposed to serum for 48 hr surface area increased 3.69 fold, but the maximum number of binding sites ([125I]-iodocynanopindolol) only increased 1.5 fold from 12956 +/- 7579 to 19676 +/- 5181 sites/cell (n = 5, p less than .05) yielding a value of 2.48 sites/um2 for cells grown in serum-supplemented medium compared with 6.96 sites/um2 for cells grown in serum-free medium. Thus serum-induced hypertrophy is associated with a decrease in beta 1 receptor density relative to cell size; however, adenylate cyclase response is unaffected. This cell culture system constitutes an excellent model for studying interventions that may influence the regulation of cardiac myocyte hypertrophy by nonhemodynamic factors, particularly through the adrenergic receptor system. |
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