Structure, speciation, DNA binding and nuclease activity of two bipyridyl-zinc complexes bearing trimethylaminomethyl groups |
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| Authors: | Ji-Hui Li Li-Yi Zhang Zong-Wan Mao Liang-Nian Ji |
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| Institution: | a MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, China
b State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002, China |
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| Abstract: | The Zn(II) complexes of two bipyridyl derivatives with trimethylaminomethyl groups, Zn(L1)3](ZnBr4)(ClO4)2Br4 · 12H2O (1) and Zn(L2)3](ClO4)8 · H2O (2) (L1 = 4,4′-(Me3NCH2)2-bpy]2+, L2 = 5,5′-(Me3NCH2)2-bpy]2+ and bpy = 2,2′-bipyridyl), were synthesized and characterized. The structure of complex 1 has been determined by X-ray diffraction, and the configuration of ligand-coordinated Zn(II) ion can be well described as distorted octahedral. Species distribution of both complexes in aqueous solution was investigated by potentiometeric titration. DNA-binding ability of both complexes was studied by UV spectroscopy titration. Under hydrolytic condition, both complexes (150 μM) can effectively cleave plasmid pBR322 DNA from supercoiled to nicked form, giving hydrolysis rate constants of 2.63 × 10−5 and 9.92 × 10−5 s−1, respectively (pH 7.75, 37 °C). The result shows that complex 2 is one of the most active artificial nucleases based on zinc. |
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| Keywords: | Zinc complexes Bipyridyl Trimethylaminomethyl groups Artificial nucleases DNA cleavage |
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