Enhancing the separation of phosphorylated proteins in gel electrophoresis with dinuclear bispyridylmethylamine-tyrosine-acrylamide complexes |
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| Authors: | Georg Dirscherl Wolfgang Seufert |
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| Affiliation: | a Institut für Organische Chemie, Universität Regensburg, D-93040 Regensburg, Germany
b Institut für Biochemie, Genetik und Mikrobiologie, Universität Regensburg, D-93040 Regensburg, Germany |
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| Abstract: | Bispyridylmethylamine-tyrosine-acrylamide ligands were prepared from protected tyrosine and dipyridylmethylamine by a twofold Mannich reaction and converted into acryl amides. Their manganese complexes were used as gel additives to increase the specific phosphate affinity in SDS-PAGE protein separations. The modified gel showed a distinct mobility shift of phosphorylated α-casein in comparison to the dephosphorylated protein. |
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| Keywords: | Coordination compounds Zinc(II) complexes Manganese(II) complexes Phosphoproteins Acryl amides SDS gel electrophoresis |
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