Effects of acid stress on polyamine levels,ion efflux,protective enzymes and macromolecular synthesis in cereal leaves |
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Authors: | Hui-Juan Shen Yin-Feng Xie Ru-Tie Li |
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Affiliation: | (1) Department of Forestry, Nanjing Forestry University, Nanjing, 210037, China |
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Abstract: | Wheat or oat leaf segments were floated on acid solutions and the changes in several parameters measured. The putrescine content of wheat leaves was 12.8-fold greater at pH 3.5 than at pH 6 after 72 h. The increase in putrescine was accompanied by an increase in the amount of K+ efflux from the leaf tissue to the external solution. The activities of superoxide dismutase and catalase of wheat leaves at pH 3.5 decreased to 51% and 40%, respectively, of their levels at pH 6 by 24 h. [3H]Uridine and [3H]leucine incorporation into macromolecules in oat leaves (with the lower epidermis removed) decreased to 58% and 28% of the control in response to acid stress in 8 h, at the same time as a 5-fold increase in putrescine. When DL- -difluoro-methyarginine was added to the pH 3.5 buffered solution, the effect of acid was slightly less, with the incorporation into macromolecules being 64% and 35% of the pH 6 control. The results indicated that the putrescine accumulation under acid stress was concomitant with ion efflux, and a decrease in both macromolecular synthesis and the activities of superoxide dismutase and catalase in cereal leaves.Abbreviations Put putrescine - Spd spermidine - Spm spermine - SOD superoxide dismutase - CAT catalase - ADC arginine decarboxylase - DFMA DL- -difluoromethylarginineThis paper is dedicated to Dr. A.W. Galston in honor for his great achievements and in recognition of his continuing contributions to the study of plant science. |
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Keywords: | polyamine acid stress superoxide dismutase catalase macromolecular synthesis putrescine |
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