粳稻三体的染色体G—显带鉴定

采用有丝分裂染色体的G-显带技术,对供试的6个三体的额外染色体进行了鉴定。结果表明:A型、B型、C型、D型、E型和H型三体的额外染色体分别为K5、K6、K12、K7、K8和K10。用不同的分裂时期的细胞所鉴定出的结果完全一致。与过去所用的三体鉴定的方法相比较,利用G-显带技术鉴定三体的方法具有准确性高,稳定性和重复性好以及简便易行的优点。

Identification of Trisomics in Japonica Rice Plants by Chromosome G-banding

Six types of trisomics were identified by the G-banding karyotype analysis. Trisomic types A, B, C, D, E and H were confirmed to have individual extra chromosomes K5, K6, K12, K7, K.8 and K10 respectively. The same results were obtained when we observed the cells of different mitotic stages. According to the chromosome G-banding patterns, the extra chromosomes were identified more easily by G-banding pattern analysis than by pachytene chromosome analysis. Using this technique high accuracy, high as well as simplicity and convenience could be achieved for identifying extra chromosomes in trisomics.