| 万古霉素的磁性亲和吸附分离 |
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| 引用本文： | 郭立安, 朱宝泉, 陈代杰,. 万古霉素的磁性亲和吸附分离[J]. 生物工程学报, 2001, 17(5): 584-586 |
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| 作者姓名： | 郭立安 朱宝泉 陈代杰 |
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| 作者单位： | 1. 第四军医大学化学教研室,
2. 上海医药工业研究院, |
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| 基金项目： | 国家自然科学基金(3008007),陕西省自然科学基金的(99H09)和高等学校骨干教师计划(2000-65-66)资助. |
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| 摘 要： | 磁性亲和分离技术是近年新兴的一个分离方法,它可直接从初始样品液体中(无论是浑浊或清亮液)分离目标产物,克服了传统色谱方法需要离心和过滤除杂质的步骤,分离过程所用仪器极其简单,大大降低了操作费用,且易于实现规模化;此外,同亲和色谱一样也具有高的特异性及应用范围广等优点.使其在蛋白质、细胞的分离方面表现出了巨大的应用前景[1-4].万古霉素是一个多肽类的抗菌素,临床上用于治疗耐甲氧苯青霉素葡萄糖球菌的感染.目前虽有多种纯化方法[5,],但大多工艺复杂,回收率较低,尤其是在纯化过程中,万古霉素极易降解,迫使人们寻求更好的纯化方法来解决这个问题.
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| 关 键 词： | 万古霉素 纯化 磁性分离 亲和色谱 |
| 文章编号： | 1000-3061(2001)05-0584-03 |
| 修稿时间： | 2001-02-07 |
Purification of Vancomycin by Using Magnetic Affinity Technique |
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| L A Guo,B Q Zhu,D J Chen. Purification of Vancomycin by Using Magnetic Affinity Technique[J]. Chinese journal of biotechnology, 2001, 17(5): 584-586 |
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| Authors: | L A Guo B Q Zhu D J Chen |
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| Affiliation: | Chemical Department, Fourth Military Medical University, Xi'an 710032, China. guoli-an@263.net |
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| Abstract: | A new kind of magnetic affinity microspheres (MAMS), whose ligand is D-Ala-D-Ala, was prepared using agarose as matrix. By using this new MAMS vancomycin was purified directly from crude fermentation liquor with only one step. The purity and the mass recovery of vancomycin measured by reverse-phase HPLC were 97% and 87%, respectively. The characteristic of this method was simpler, faster, cheaper and more effective than that of currently used ones. |
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| Keywords: | vancomycin purification magnetic separation affinity chromatography |
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