Activity of a maize ubiquitin promoter in transgenic rice |
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| Authors: | Maria-Jesús Cornejo Diane Luth Kathleen M Blankenship Olin D Anderson Ann E Blechl |
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| Institution: | (1) Western Regional Research Center, Agricultural Research Service, United States Department of Agriculture, 800 Buchanan Street, 94710 Albany, CA, USA;(2) Present address: Departmento de Biologia Vegetal, Facultad de Farmacia, Universidad de Valencia, Avda. Vicente Andres Estelles s/n, 46100 Burjassot, Valencia, Spain;(3) Present address: Department of Fruit Crops, University of Florida, 32611 Gainesville, FL, USA;(4) Present address: Neurogenetics Laboratory, University of California, 94143 San Francisco, CA, USA |
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| Abstract: | We have used the maize ubiquitin 1 promoter, first exon and first intron (UBI) for rice (Oryza sativa L. cv. Taipei 309) transformation experiments and studied its expression in transgenic calli and plants. UBI directed significantly higher levels of transient gene expression than other promoter/intron combinations used for rice transformation. We exploited these high levels of expression to identify stable transformants obtained from callus-derived protoplasts co-transfected with two chimeric genes. The genes consisted of UBI fused to the coding regions of the uidA and bar marker genes (UBI:GUS and UBI:BAR). UBI:GUS expression increased in response to thermal stress in both transfected protoplasts and transgenic rice calli. Histochemical localization of GUS activity revealed that UBI was most active in rapidly dividing cells. This promoter is expressed in many, but not all, rice tissues and undergoes important changes in activity during the development of transgenic rice plants. |
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| Keywords: | GUS expression heat shock Oryza sativa L rice transformation ubiquitin |
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