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Chromosomal DNA patterns and mitochondrial DNA polymorphism as tools for identification of enological strains of Saccharomyces cerevisiae |
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| Authors: | Françoise Vezinhet Bruno Blondin Jean-Noel Hallet |
| Affiliation: | (1) Laboratoire de Microbiologie et Technologie des Fermentations, Institut des Produits de la Vigne, INRA, 2 place Viala, 34060 Montpellier Cédex, France;(2) Chaire de Technologie Alimentaire et Oenologie, Institut des Produits de la vigne, ENSAM, 2 place Viala, 34060 Montpellier Cédex, France;(3) Laboratoire de Cytophysiologie végétale, Université de Nantes, 2 rue de la Houssinière, 44072 Nantes Cédex 03, France |
| Abstract: | Summary Chromosomal DNA patterns using the transverse alternating field electrophoresis technique and mitochondrial DNA restriction profiles have been achieved for 22 enological strains of Saccharomyces cerevisiae. Both methods have evidenced a marked polymorphism of these strains. Twenty different karyotypes and 17 mitochondrial DNA banding patterns have been observed. Only three strains originating from the same vineyard could not be differentiated by either of the two methods. The polymorphism observed at the chromosomal and mitochondrial levels makes the techniques investigated powerful tools for identification and control of industrial strains.Offprint requests to: J.-N. Hallet |
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