Rabbit antibodies against Toxoplasma Hsp20 are able to reduce parasite invasion and gliding motility in Toxoplasma gondii and parasite invasion in Neospora caninum |
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| Authors: | Veronica M Coceres Andrés M Alonso M Lis Alomar María M Corvi |
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| Institution: | 3. Institute of Parasitology, Biology Centre of the Academy of Sciences of the Czech Republic, ?eské Budějovice, CZ 370 05, Czech Republic;4. Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Prague, CZ 16610, Czech Republic;6. Sandler Center for Drug Discovery, University of California, San Francisco, California 94158;5. Department of Pharmaceutical Chemistry, University of California, San Francisco, California 94720;1. Centre National de la Recherche Scientifique (CNRS), Unité Mixte de Recherche 5164, 33000 Bordeaux, France;2. University of Bordeaux, Bordeaux, France;3. Institute of Biology, Leiden University, Einsteinweg 55, 2333 CC Leiden, The Netherlands;1. Clinic for Vascular and Endovascular Surgery, Clinical Center of Serbia, Belgrade, Serbia;2. School of Medicine, University of Belgrade, Serbia;1. Laboratory of Molecular and Evolutionary Parasitology, RAPID Group, School of Biosciences, University of Kent, Canterbury, Kent, UK;2. School of Biosciences, University of Kent, Canterbury, Kent, UK |
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| Abstract: | Toxoplasma gondii Hsp20 is a pellicle-associated functional chaperone whose biological role is still unknown. Hsp20 is present in different apicomplexan parasites, showing a high degree of conservation across the phylum, with Neospora caninum Hsp20 presenting an 82% identity to that of T. gondii. Hence rabbit anti-T. gondii Hsp20 serum was able to recognize the N. caninum counterpart. Interestingly, both N. caninum and T. gondii Hsp20 localized to the inner membrane complex and to the plasma membrane. Incubation of T. gondii and N. caninum tachyzoites with an anti-TgHsp20 serum reduced parasite invasion at rates of 57.23% and 54.7%, respectively. This anti-serum also reduced T. gondii gliding 48.7%. Together, all this data support a role for Hsp20 in parasite invasion and gliding motility. |
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