Enhanced production and secretion of streptokinase into extracellular medium in Escherichia coli by removal of 13 N-terminal amino acids |
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| Authors: | Si Hyoung Lee Il Chul Kim Kwang Hee Bae Si Myung Byun |
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| Institution: | (1) Department of Biological Sciences, Korea Advanced Institute of Science and Technology and Research Center for New Bio-Materials in Agriculture, 373-1, Kusong-dong, Yusong-gu Taejon, 305-701, Korea |
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| Abstract: | The production and secretion of streptokinase using OmpA signal sequence in E. coli was enhanced by removing the 13 N-terminal amino acids (SK(N13). The secretion level of SK(N13 protein into the extracellular medium was two times higher than that of wild-type streptokinase. About 4500 IU of SK(N13 protein per 1 ml LB-ampicillin medium was secreted into extracellular medium at 12 hours after induction. Fully active and enhanced extracellular preparation of the mutant streptokinase may be a potential alternative source for the simple downstream processing |
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