Quantification and Modeling of Plasmid Mobilization on Seeds and Roots |
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Authors: | Padma Sudarshana Guy R Knudsen |
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Institution: | (1) Soil and Land Resources Division, Department of Plant, Soil, and Entomological Sciences, University of Idaho, Moscow, Idaho 83844-2339, USA;(2) Present address: University of California, Davis, CA 95616, USA |
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Abstract: | Mobilization frequencies of the nonconjugative plasmid pMON5003 were quantified using Escherichia coli TB1(pRK2013) as donor of a helper plasmid, E. coli M182 (pMON5003) as donor of the nonconjugative plasmid, and Pseudomonas fluorescens as recipient. Initial mating experiments were conducted in nutrient and minimal salts media and pea seed exudates. Mobilization
rates were higher during early stationary growth of donors, helpers, and recipients. Numbers of transconjugants were higher
in biparental matings when donors contained both conjugative and nonconjugative plasmids, versus tri-parental matings. A mathematical
model was developed to predict a nonconjugative plasmid transfer rate parameter (δ), estimating the proportion of conjugative
matings in which a plasmid is mobilized. Values of δ ranged from 8 × 10−3 to 7.9 × 10−1. Transfer frequencies for pMON5003 from E. coli to P. fluorescens on pea seeds and roots were determined. Transconjugants (P. fluorescens 2-79 (pMON5003)) were isolated from seeds, roots, and soil, but mobilization frequencies were lower than in liquid media. |
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