马尾松成熟合子胚的体细胞胚胎发生和植株再生

采用马尾松（Ｐｉｎｕｓｍ ａｓｓｏｎｉａｎａ Ｌａｍ ｂ．）成熟合子胚为起始外植体，在含２，４－Ｄ １０ ｍ ｇ／Ｌ，ＫＴ和ＢＡ 各４ ｍ ｇ／Ｌ的ＤＣＲ培养基上得到胚发生培养物。将白色半透明的愈伤组织（含早期原胚）在含２，４－Ｄ１．０ ｍ ｇ／Ｌ，ＫＴ和ＢＡ 各０．４ ｍ ｇ／Ｌ的ＤＣＲ培养基上保持并增殖。在附加９０００ ｍ ｇ／Ｌ肌醇的ＤＣＲ高渗培养基上得到粗壮的后期原胚。ＡＢＡ 和活性炭同时使用能促进子叶胚的形成，最高频率为３５．１％ 。在无激素培养基上，成熟体细胞胚萌发并进一步形成完整小植株

SOMATIC EMBRYOGENESIS AND PLANTLET REGENERA TION FROM CALLUS OF MATURE ZYGOTIC EMBRYOS OF MASSON PINE

Embryogenic cultures were initiated from mature zygotic embryos of masson pine (Pinus massoniana Lamb.) on DCR medium supplemented with 2,4 D 10 mg/L, KT and BA each at 4 mg/L. Pale and translucent calli with early stage proembryos were maintained and multiplicated on DCR medium supplemented with 2,4 D 1 0 mg/L KT and BA each at 0 4 mg/L.Robust late stage proembryos were obtained when the calli were cultured on DCR medium containing 9000 mg/L myo inositol.Abscisic acid and activated charcoal promoted the formation of cotyledonary embryos at the highest frequency of 35 1%.Mature somatic embryos could germinate and develop further into plantlets when they were isolated and cultured on a hormone free DCR medium.