烟草传粉前后生殖器官中细胞分裂素含量的变化

经免疫亲和层析系统纯化后，用间接竞争ＥＬＩＳＡ 法测定了烟草（Ｎｉｃｏｔｉａｎａ ｔａｂａｃｕｍ ）生殖器官在传粉前后细胞分裂素（ｔ－ＺＲ，ｉＰＡ）含量的变化． 开花前５ ｄ，花药和花丝中的细胞分裂素（ＣＴＫ）含量均达到最高值，以后随雄蕊发育逐渐下降． 授粉使花柱ＣＴＫ 含量急剧上升，授粉后１ ｄ 达到高峰，未经受粉的花柱ＣＴＫ 开花后下降．授粉后２ ｄ，子房中的ＣＴＫ 开始上升，在授粉后４ ｄ 达到最高值，而未受精的子房ＣＴＫ 含量开花后下降． 传粉后雌蕊中ＣＴＫ含量随花粉管生长而有规律地增加，ＣＴＫ 积极参与植物的传粉和受精过程

CHANGE IN CYTOKININ LEVELS IN REPRODUCTIVE ORGANS OF NICOTIANATABACUM BEFORE AND AFTER POLLINATION

Immunoaffinity systems and indirect competitive ELISA were used to determine the changes of cytokinin (CTK) content in reproductive organs of Nicotiana tabacum before and after pollination. It was found that t ZR and iPA existed in all reproductive organs tested, and their contents were higher in female than in male organs. On the 5th day before anthesis, CTK reached its peak value in anthers and filaments, then it declined. In styles, CTK content increased after pollination and reached its peak one day later. In ovaries, levels of CTK began to increase two days after pollination and reached its peak two days later, just at the time when fertilization took place. If unpollinated, CTK levels decreased both in styles and ovaries. Pollination and fertilization resulted in an increment of CTK in pistils, accompanied with the process of pollen germination and pollen tube growth. It could thus be inferred that t ZR and iPA played some active role in pollination and fertilization.