Design of recombinant antibody microarrays for membrane protein profiling of cell lysates and tissue extracts |
| |
Authors: | Dexlin-Mellby Linda Sandström Anna Antberg Linn Gunnarsson Jenny Hansson Stefan R Borrebaeck Carl A K Wingren Christer |
| |
Institution: | Department of Immunotechnology, BMC D13, Lund University, Lund, Sweden. |
| |
Abstract: | Generating global protein expression profiles, including also membrane proteins, will be crucial for our understanding of biological processes in health and disease. In this study, we have expanded our antibody microarray technology platform and designed the first human recombinant antibody microarray for membrane proteins targeting crude cell lysates and tissue extracts. We have optimized all key technological parameters and successfully developed a setup for extracting, labeling and analyzing non-fractionated membrane proteomes under non-denaturing conditions. Finally, the platform was also extended and shown to be compatible with simultaneous profiling of both membrane proteins and water-soluble proteins. |
| |
Keywords: | Antibody microarrays Cell lysate Membrane proteins Membrane proteomics Protein arrays |
本文献已被 PubMed 等数据库收录! |
|