CHANGES IN ACTIVITY OF DGLUCOSE-6-PHOSPHATE: NADP AND 6-PHOSPHO-D-GLUCONATE: NADP OXIDOREDUCTASES IN RELATION TO LIGNIFICATION OF BAMBOO |
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Authors: | HIGUCHI, TAKAYOSHI SHIMADA, MIKIO |
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Affiliation: | Laboratory of Wood Chemistry, Faculty of Agriculture, Gifu University Kakamigahara, Gifu |
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Abstract: | D-Glucose-6-phosphate: NADP oxidoreductase (glucose-6-phosphatedehydrogenase; EC 1.1.1.49[EC]) and 6-phospho-D-gluconate: NADPoxidoreductase (6-phosphogluconate dehydrogenase; EC 1.1.1.44[EC])were found to be present in immature bamboo. Optimal pHs ofthe glucose-6-phosphate- and 6-phosphogluconate dehydrogenaseswere found to be 8.0 and 8.5, respectively. Both enzymes were demonstrated to be NADP-specific and NADPcould not be replaced by NAD. Fructose-6-phosphate was indirectlyutilized after convrsion to glucose-6-phosphate by glucose-6-phosphateisomerase coexisting in the enzyme preparation. Pattern of enzyme activity and of respiratory breakdown of glucose-1-14Cand glucose-6-14C were investigated in connection with lignificationof bamboo and discussed in comparison with sugar metabolismof fungi-infected plant tissues. As for the changes in the enzymeactivity with growth of bamboo, it was recognized that therewas a tendency that the activity of both enzymes increased andwas maintained at a certain level even in the aged tissues.In addition there was a drop of the C6/C1 ratio toward the tissuesof lower parts containing considerable amount of lignin andthis phenomenon was the same as that observed in pentose phosphatemetabolism of fungi-infected plant tissues. (Received September 5, 1966; ) |
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