Enterobacter aerogenes OmpX, a cation-selective channel mar- and osmo-regulated |
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Authors: | Dupont Myrielle Dé Emmanuelle Chollet Renaud Chevalier Jacqueline Pagès Jean-Marie |
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Affiliation: | EA2197, IFR48, Faculté de Médecine, Université de la Méditerranée, 27 Bd Jean Moulin, 13385 Marseille Cedex 05, France. |
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Abstract: | The ompX gene of Enterobacter aerogenes was cloned. Its overexpression induced a decrease in the major porin Omp36 production and consequently a beta-lactam resistance was noted. Purified outer membrane protein X (OmpX) was reconstituted into artificial membranes and formed ion channels with a conductance of 20 pS in 1 M NaCl and a cationic selectivity. Both MarA expression and high osmolarity induced a noticeable increase of the OmpX synthesis in the E. aerogenes ATCC 13048 strain. In addition, OmpX synthesis increased under conditions in which the expression of the E. aerogenes major non-specific porins, Omp36 and Omp35, decreased. |
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