Effect of cyclodextrin on the activity and secondary structure of horseradish peroxidase |
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Authors: | Chen Jian Bo Xu Yi Xia Chun Gu Li Shu Ben |
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Institution: | College of Life and Environment Science, Shanghai Teachers University, Shanghai 200234, P.R. China. |
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Abstract: | The activity and secondary structure of horseradish peroxidase (HRP) was studied in aqueous solution containing alpha-, beta- and gamma-cyclodextrin (CD). The results showed that the activity of HRP was enhanced to different extents by the three kinds of CD. A Fourier Transform infrared (FTIR) spectroscopy study indicated that the amount of alpha-helical structure was important for the activity of HRP. This phenomenon is discussed. |
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