Allosteric effect of protons and adenosine triphosphate on hemoglobins from aquatic amphibia |
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| Authors: | Amora A B Lima Nilce C Meirelles Lucia P S Airoldi A Focesi Jr |
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| Institution: | (1) Departamento de Bioquimica, Instituto de Biologia, Universidade Estadual de Campinas, CP. 1170, 13100 Campinas, SP., Brazil;(2) Present address: Hostpital das Clinicas, Universidade Federal do Rio Grande do Norte, Natal, RN., Brazil |
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| Abstract: | Summary The analysis of hemoglobins from two anurans, one semi-aquatic (Leptodactylus labyrinthicus) and the other aquatic (Pipa carvalhoi), showed several components isolated by CM-cellulose chromatography. The two major components (Hb II and Hb III) ofL. labyrinthicus and the major components (Hb III and Hb IV) ofP. carvalhoi possess functional properties as follows: i.P. carvalnoi Hb III and Hb IV andL. labyrinthicus Hb II had very small Bohr effects (–0.05) and a substantial heterotropic effect with polyphosphates. ii.L. labyrinthicus Hb III produced a normal Bohr effect of –0.17, with no influence of polyphosphates.The authors wish to dedicate this paperin memoriam of Prof. Eraldo Antonini who contributed much to the knowledge of hemoglobins |
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