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一株高效解无机磷细菌BS06的鉴定及其解磷能力分析
引用本文:史国英,莫燕梅,岑贞陆,曾泉,余功明,杨丽涛,胡春锦. 一株高效解无机磷细菌BS06的鉴定及其解磷能力分析[J]. 微生物学通报, 2015, 42(7): 1271-1278
作者姓名:史国英  莫燕梅  岑贞陆  曾泉  余功明  杨丽涛  胡春锦
作者单位:1. 广西农业科学院微生物研究所 广西 南宁 530007;2. 广西作物遗传改良生物技术重点开放实验室 广西 南宁 530007,3. 广西大学 广西 南宁 530004;4. 贺州学院 广西 贺州 542800,1. 广西农业科学院微生物研究所 广西 南宁 530007,1. 广西农业科学院微生物研究所 广西 南宁 530007,3. 广西大学 广西 南宁 530004,1. 广西农业科学院微生物研究所 广西 南宁 530007;3. 广西大学 广西 南宁 530004,1. 广西农业科学院微生物研究所 广西 南宁 530007;2. 广西作物遗传改良生物技术重点开放实验室 广西 南宁 530007
基金项目:国家自然科学基金项目(No. 31101122);广西自然科学基金项目(No. 2011GXNSFA018076);广西农科院基金项目(No. 2013YT04,2013JQ09,2012GW06);“广西特聘专家”专项项目
摘    要:【目的】对一株来源于广西甘蔗根际土壤的高效解无机磷细菌BS06的分类和解磷能力进行探讨,以期为解磷微生物在广西甘蔗生产上的开发和应用提供理论依据。【方法】通过形态观察、生理生化测定及16S rRNA基因序列同源性分析,进一步结合种特异的recA基因序列分析对解磷菌BS06进行分类鉴定;通过改变无机磷培养基中的碳源、氮源对菌株解磷能力的影响,分析菌株的解磷特性;通过盆栽试验了解菌株对甘蔗品种粤糖00236、桂糖28磷素吸收的影响。【结果】分类鉴定结果表明菌株BS06属于洋葱伯克霍尔德菌(Burkholderia cepacia);菌株在以乳糖为碳源条件下具有较强的解磷能力,其发酵液中水溶性磷含量为262.71 mg/L;在以硝酸钠为氮源条件下有较强解磷能力,其发酵液中水溶性磷含量达到305.85 mg/L;接种BS06菌株显著促进甘蔗组培苗的生长并提高甘蔗植株的含磷量。【结论】解磷细菌BS06具有较大的开发利用潜力。

关 键 词:甘蔗,解磷细菌,鉴定,解磷能力

Identification of an inorganic phosphorus-dissolving bacterial strain BS06 and analysis on its phosphate solubilization ability
SHI Guo-Ying,MO Yan-Mei,CEN Zhen-Lu,ZENG Quan,YU Gong-Ming,YANG Li-Tao and HU Chun-Jin. Identification of an inorganic phosphorus-dissolving bacterial strain BS06 and analysis on its phosphate solubilization ability[J]. Microbiology China, 2015, 42(7): 1271-1278
Authors:SHI Guo-Ying  MO Yan-Mei  CEN Zhen-Lu  ZENG Quan  YU Gong-Ming  YANG Li-Tao  HU Chun-Jin
Affiliation:1. Institute of Microbiology, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi 530007, China; 2. Guangxi Crop Genetic Improvement and Biotechnology Laboratory, Nanning, Guangxi 530007, China,3. Guangxi University, Nanning, Guangxi 530004, China; 4. Hezhou University, Hezhou, Guangxi 542800, China,1. Institute of Microbiology, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi 530007, China,1. Institute of Microbiology, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi 530007, China,3. Guangxi University, Nanning, Guangxi 530004, China,1. Institute of Microbiology, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi 530007, China; 3. Guangxi University, Nanning, Guangxi 530004, China and 1. Institute of Microbiology, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi 530007, China; 2. Guangxi Crop Genetic Improvement and Biotechnology Laboratory, Nanning, Guangxi 530007, China
Abstract:[Objective] The purpose of the present study was to investigate the phosphate-solubilizing ability of a bacterial stain BS06, for developing and applying effectively phosphate-solubilizing microbes in sugarcane production in Guangxi. [Methods] 16S rRNA gene sequence homology comparison, recA gene analysis as well as morphological, physiological and biochemical analyses were carried out to identify the bacterial strain BS06. The phosphate-solubilizing ability of BS06 was investigated under the conditions of changing the carbon and nitrogen source, separately in inorganic phosphate medium. [Results] The strain BS06 was identified as Burkholderia cepacia. The strain had high and efficient phosphate-solubilizing ability under the condition of carbon source with lactose and nitrogen source with sodium nitrate separately, and the concentration of water soluble phosphorus in fermentation solution was 262.71 mg/L and 305.85 mg/L, respectively. Compared with the uninoculated controls, BS06 significantly increased dry weights and phosphorus contents of micropropagated sugarcane seedlings. [Conclusion] The bacterial strain BS06 had high potential of exploitation.
Keywords:Sugarcane   Phosphate-solubilizing bacteria   Identification   Phosphate-solubilizing ability
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