A hyperconversion mechanism generates the chicken light chain preimmune repertoire |
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| Authors: | C A Reynaud V Anquez H Grimal J C Weill |
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| Institution: | 1. Department of Biology, The College of William and Mary, Williamsburg, VA 23185, USA;2. Department of Environmental and Aquatic Animal Health, Virginia Institute of Marine Science, The College of William and Mary, Williamsburg, VA 23185, USA;3. National Center for Cool and Cold Water Aquaculture, Agricultural Research Service, USDA, Kearneysville, WV 25430, USA |
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| Abstract: | The chicken immunoglobulin light chain repertoire has been shown to be entirely derived from a single V lambda 1-J rearranged combination. The complete coding information of the lambda locus was determined: it comprises 25 V-hybridizing elements, all of which are pseudogenes, clustered in both orientations within 19 kb of DNA, starting 2.4 kb upstream of the V lambda 1 gene. Sequences of somatically rearranged V lambda 1 genes from embryonic and posthatching bursal cells show that diversification of light chain sequences occurs during ontogeny by a segmental gene conversion mechanism which takes place at a frequency of 0.05-0.1 per cell generation between the pseudogene pool and the unique rearranged functional V gene. |
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