Supercritical carbon dioxide extraction,fluorimetric and electrochemical high performance liquid chromatrographic detection of vitamin E from Hordeum vulgare L. |
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| Authors: | M. L. Colombo A. Corsini A. Mossa L. Sala M. Stanca |
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| Affiliation: | 1. Institute of Pharmacological Sciences, University of Milan, Via Balzaretti 9, 20133 Milano, Italy;2. Experimental Institute for Cereal Research, Via S.Protaso, Fiorenzuola d'Arda, Piacenza, Italy |
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| Abstract: | A supercritical fluid extraction (SFE), method has been applied to extract the natural vitamin E components, tocopherols and tocotrienols, from fruits of Hordeum vulgare L. The fractions were monitored by analytical high performance liquid chromatography using fluorimetric and electrochemical detectors. High yields, and clear and purified extracts were obtained by SFE; compared with the n-hexane extract, the SFE extract was lighter in colour and more concentrated in vitamin E components. © 1998 John Wiley & Sons, Ltd. |
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| Keywords: | supercritical carbon dioxide vitamin E Hordeum vulgare L caryopsis tocopherols tocotrienols high performance liquid chromatography |
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