A <Emphasis Type="Italic">Bacillus subtilis</Emphasis> strain HPC248 from an effluent treatment plant with antimicrobial activity |
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Authors: | Atya Kapley Sameera N Siddiqui Hemant J Purohit |
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Institution: | (1) Environmental Genomics Unit, National Environmental Engineering Research Institute, Nehru Marg, Nagpur, 440 020, India |
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Abstract: | This study reports the identification and demonstration of an organism with antimicrobial activity isolated from activated
biomass of an effluent treatment plant (ETP) treating wastewater containing pesticides. While assessing the heterotrophic
diversity of biomass collected from ETP, clear zones were observed on Luria Bertani plates. The bacterial isolate producing
the zone as well as the bacterial cells surrounding the zone were isolated and purified by sub-culturing. Both isolates were
identified by partial sequencing of the 16S rDNA clone. Presence of antimicrobial activity was demonstrated against various
laboratory strains, isolated from different treatment plants and also against waterborne pathogens. The isolate that produced
antimicrobial activity was identified as Bacillus subtilis strain HPC248 and the sensitive strain was identified as Bacillus sphaericus strain HPC249. |
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Keywords: | antimicrobial activity 16S rDNA Bacillus partial sequencing activated biomass |
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