In Vitro Characterization of a Recombinant AHL-Lactonase from Bacillus cereus Isolated from a Striped Catfish (Pangasianodon hypophthalmus) Pond |
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Authors: | Nguyen Thi Ngoc Tinh Nguyen Viet Dung Cao Thanh Trung Van Thi Thuy |
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Affiliation: | 1. Research Institute for Aquaculture No. 2, 116 Nguyen Dinh Chieu, District 1, Ho Chi Minh City, Vietnam
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Abstract: | aiiA gene encoding AHL-lactonase was isolated from Bacillus cereus strain N26.2, originating from a striped catfish (Pangasianodon hypophthalmus) pond in Vietnam. This gene, abbreviated as aiiA(N26.2), was cloned and expressed in a competent Escherichia coli strain BL21(DE3)pLysS. The resulting protein, abbreviated as AiiAN26.2, was highly active in the pH range of 6–8 and could retain 80 % of the maximum activity under storage for 5 days at 4 °C or for 3 days at 20 °C. These properties of AiiAN26.2 protein confers its future application via feed supplementation, with the purpose of controlling aquaculture pathogens which regulate the virulence via a quorum sensing system. |
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Keywords: | AHL-lactonase activity AHL-mediated quorum sensing aiiA gene Bacillus cereus Recombinant AHL-lactonase |
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