Reaction of common bean callus to culture filtrate of Colletotrichum lindemuthianum: Differences in the composition and toxic activity of fungal culture filtrates |
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Authors: | Fernández M T Fernández M Centeno M L Cañal M J Rodriguez R |
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Institution: | (1) Laboratorio de Fisiología Vegetal, Universidad de Oviedo, Spain;(2) Present address: Laboratorios M, C/ Marqués de Teverga, 7. Esc. Izda. 3° C, 33005 Oviedo, Asturias, Spain |
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Abstract: | Culture filtrate of Colletotrichum lindemuthianum caused dark brown lesions on the lower surface veins of bean leaves. This phenomenon was used as a bioassay to study the
production of toxic fungal metabolites. Calli from anthracnose-susceptible bean cultivars 'Collacia', 'Andecha' and 'Seronda'
were sensitive to a 12.5% solution of race 38 filtrate or to a 25% solution of race 7 filtrate. In contrast, calli from anthracnose-resistant
bean genotypes A 247, TU, PI 207262, 'Collacia' × 'Tu', 'Collacia' × AB 136 and 'Collacia' × PI 207262 did not develop browning.
Culture filtrates were passed through an ionic-exchange resin and a gel filtration resin. Toxic activity of fractions from
two races of the fungal pathogen was different, although in both races slight necrosis was produced by the same nine fractions.
Pathogenicity could be related with common substances and toxicity could be identified with differential compounds.
This revised version was published online in June 2006 with corrections to the Cover Date. |
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Keywords: | anthracnose culture filtrate disease resistance in vitro selection Phaseolus vulgaris pathotoxin |
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