Properties and characterization of low molecular weight inhibitor of calmodulin-dependent cAMP phosphodiesterase from rat liver |
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Authors: | R H Rao M Palazzolo L Sanders J A Smoake S S Solomon |
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Affiliation: | Research Service and Department of Medicine Veterans Administration Medical Center and University of Tennessee Center for the Health Sciences, 1030 Jefferson Avenue, Memphis, TN 38104, USA |
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Abstract: | We have identified two different species of inhibitors of calmodulin-dependent cAMP phosphodiesterase: 1) a low molecular weight (LMW) and 2) a high molecular weight (HMW) form. These inhibitors are extracted from rat liver. Both LMW and HMW inhibitors are heat-stable, acidic in nature and lose activity with prolonged storage and/or repeated freezing and thawing. The low molecular weight inhibitor has been purified to about 7,000-fold with 300% recovery. LMW inhibits calmodulin-dependent cAMP phosphodiesterase regardless of the source of calmodulin (e.g. fat, brain, heart, erythrocytes). LMW appears to be lipid in nature with a molecular weight of 1,500-5,000. The role of these inhibitors in diabetes and mechanism of action of insulin is presented. |
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Keywords: | To whom reprint request should be addressed |
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