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Electrotransformation of germinating spores of Streptomyces spp.
Authors:M Tyurin  L Starodubtseva  H Kudryavtseva  T Voeykova  V Livshits
Institution:(1) Russian State Research Centre GNIIGENETIKA, 1st Dorozhny Pr.1, 113545 Moscow, Russia
Abstract:Summary Germinating spores of Streptomyces lividans, S. aureofaciens, S. rimosus and S. virginiae were electrotransformed with pIJ699, pIJ702, pWOR109 and pZAT22 plasmid DNAs. In all cases, thiostrepton resistant transformants were obtained with an efficiency of 1 × 102 to 5 × 103 per mgrg of plasmid DNA.
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