High frequency embryoid and plantlet formation from tissue cultures of the Finger millet-Eleusine coracana (L.) Gaertn |
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Authors: | Prasanna Sivadas S L Kothari N Chandra |
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Institution: | (1) Department of Botany, University of Rajasthan, Jaipur, India;(2) Plant Genetic Manipulation Group, Department of Botany, University of Nottingham, NG7 2RD Nottingham, UK |
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Abstract: | Compact nodulated embryogenic callus differentiated from cultured seeds of Eleusine coracana (Finger Millet) on Murashige and Skoog (1962) basal medium with 2,4-dichlorophenoxyacetic acid (1.0, 3.0 mg l). This embryogenic callus was maintained on a medium with a lower level of 2,4 — dichlorophenoxyacetic acid. At every subculture the embryogenic callus had some preexisting embryoids in it. With this method of subculture the callus has retained its morphogenic potential for four years. Following transfer to media with different levels of auxins and cytokinins, the callus showed varied patterns of growth and morphogenesis. Embryoids could be germinated in profusion to form plantlets which could be transferred to the field. Shoot buds also differentiated from the whole surface of the embryoid or from the flattened meristemoids.Abbreviations BAP
6-benzylaminopurine
- IAA
indole-3-acetic acid
- 2,4-D
2,4-dichlorophenoxyacetic acid
- NAA
napthaleneacetic acid
- IBA
indolebutyric acid
- KN
kinetin
- MS
Murashige and Skoog (1962)
- GA3
Gibberellic acid |
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