Molecular cloning of cDNA for rat liver lipoate acetyltransferase a component of pyruvate dehydrogenase complex |
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| Institution: | 1. Laboratory of Cell Biology, Department of Orthopedic Surgery University Hospital of Tübingen, Waldhörnlestraße 22, 72072 Tübingen, Germany;2. Department of Orthopedic Surgery University Hospital of Tübingen, Hoppe-Seyler-Straße 3, 72076 Tübingen, Germany;3. Medical Faculty of the University of Tübingen, Geissweg 5/1, 72076 Tübingen, Germany;4. Bavarian Health and Food Authority, Veterinärstraße 2, 85764 Oberschleißheim, Germany;5. Research Center for Tissue Replacement, Regeneration & Neogenesis, Department of Orthopedics and Trauma Surgery, Faculty of Medicine, Medical Center - Albert-Ludwigs-University of Freiburg, Hugstetter Straße 55, 79106 Freiburg im Breisgau, Germany;1. Department of Biomedical Sciences, Cooper Medical School of Rowan University, Camden, NJ 08103, USA;2. Department of Chemistry & Biochemistry, Department of Molecular & Cellular Biosciences, Rowan University, Glassboro, NJ 08028, USA;1. Centro de Ciências da Saúde, Departamento de Neuropsiquiatria, Universidade Federal de Santa Maria, RS, Brazil;2. Centro de Ciências Naturais e Exatas, Programa de Pós-graduação em Ciências Biológicas: Bioquímica Toxicológica, Universidade Federal de Santa Maria, RS, Brazil;3. Centro de Educação Física e Desportos, Departamento de Métodos e Técnicas Desportivas, Laboratório de Bioquímica do Exercício (BIOEX), Universidade Federal de Santa Maria, RS, Brazil;4. Centro de Ciências da Saúde, Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria, RS, Brazil;5. UCLA, School of Medicine, Los Angeles, CA, United States |
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| Abstract: | One cDNA clone for lipoate acetyltransferase, a component enzyme of pyruvate dehydrogenase complex, was isolated from a rat liver cDNA library prepared in the phage expression vector λgt11 using immunological screening with affinity purified anti-lipoate acetyltransferase antibody. It was identified that cDNA insert in this clone codes for lipoate acetyltransferase by immunoblotting of lysogen carrying the isolated clone. Lipoate acetyltransferase antigenic polypeptide in fusion protein was about 11,000 daltons, agreeing with the size of cDNA insert to be 300 base pairs. |
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