Construction of non-polar mutants in <Emphasis Type="Italic">Haemophilus influenzae</Emphasis> using FLP recombinase technology |
| |
| Authors: | Erin Tracy Fang Ye Beth D Baker Jr" target="_blank">Robert S MunsonJr |
| |
| Institution: | (1) Center for Microbial Pathogenesis in The Research Institute at Nationwide Children's Hospital, Columbus, OH, USA;(2) The Center for Microbial Interface Biology and Department of Pediatrics, The Ohio State University, Columbus, OH, USA |
| |
| Abstract: | Background Nontypeable Haemophilus influenzae (NTHi) is a gram-negative bacterium that causes otitis media in children as well as other infections of the upper and lower
respiratory tract in children and adults. We are employing genetic strategies to identify and characterize virulence determinants
in NTHi. NTHi is naturally competent for transformation and thus construction of most mutants by common methodologies is relatively
straightforward. However, new methodology was required in order to construct unmarked non-polar mutations in poorly expressed
genes whose products are required for transformation. We have adapted the lambda red/FLP-recombinase-mediated strategy used
in E. coli for use in NTHi. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
