Comparative analysis of the replication regions of IncB, IncK, and IncZ plasmids. |
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| Authors: | J Praszkier T Wei K Siemering and J Pittard |
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| Institution: | Department of Microbiology, University of Melbourne, Parkville, Victoria, Australia. |
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| Abstract: | Minireplicons from the I-complex plasmids R387 (IncK) and pIE545 (IncZ) were constructed, and the nucleotide sequences of their replication regions were compared with that of the B plasmid, pMU720. The coding sequence of the putative replication protein, RepA, of each plasmid was located. RepA of K and B plasmids were homologous, whereas RepA of Z resembled RepA1 of FII plasmid. Sequences upstream of RepA were conserved in the three I-complex plasmids. Group B and Z plasmids were incompatible. |
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