Visualizing real time [11C]methionine translocation in Fe-sufficient and Fe-deficient barley using a Positron Emitting Tracer Imaging System (PETIS) |
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| Authors: | Nakanishi, H Bughio, N Matsuhashi, S Ishioka, N Uchida, H Tsuji, A Osa, A Sekine, T Kume, T Mori, S |
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| Affiliation: | Laboratory of Plant Molecular Physiology, Department of Applied Biological Chemistry, The University of Tokyo, 1-1-1 Yoyoi, Bunkyo-ku, Tokyo 113-8657, Japan; Takasaki Radiation Chemistry Research Establishment, Japan Atomic Energy Research Institute, Gunma 370-1207, Japan; Department of Radioisotopes, Japan Atomic Energy Research Institute, Gunma 370-1207, Japan; Central Research Laboratory, Hamamatsu Photonics Co., Shizuoka 434-0041, Japan; Corresponding author; Fax: +81 3 5684 4822; E-mail: asmori@hongo.ecc.u-tokyo.ac.jp |
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| Abstract: | [11C]methionine was supplied to Fe-deficient andFe-sufficient barley plants through a single leaf, and real time11C movement was monitored using a Positron EmittingTracer Imaging System (PETIS). In Fe-deficient plants,[11C]methionine was translocated from the tip of theabsorbing leaf to the 'discrimination centre' located at the base of theshoot, and then retranslocated to all the chlorotic leaves within 60 min,while a negligible amount was retranslocated to the roots. In Fe-sufficientplants, methionine was translocated to the discrimination centre and thenonly to the newest leaf on the main shoot within 60 min. A negligibleamount was also retranslocated to the roots. In conclusion, methionine fromthe above-ground parts of a plant is not a precursor of mugineic acid underFe-deficiency. The discrimination centre is suggested to play a vital rolein the distribution of mineral elements and metabolites in graminaceousmonocots.Keywords:[11C]methionine, discrimination centre,Fe deficiency, mugineic acid, PETIS. |
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