Differential Proteome Analysis of Hagfish Dental and Somatic Skeletal Muscles |
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Authors: | Kuo-Hsun Chiu Hurng-Wern Huang Hin-Kiu Mok |
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Institution: | (1) Institute of Marine Biology, National Sun Yat-sen University, Kaohsiung, 80424, Taiwan;(2) Institute of Biomedical Sciences, National Sun Yat-sen University, Kaohsiung, 80424, Taiwan;(3) Kaohsiung Medical University Joint Research Center, National Sun Yat-sen University, Kaohsiung, 80424, Taiwan;(4) Asia-Pacific Ocean Research Center, National Sun Yat-sen University, Kaohsiung, 80424, Taiwan |
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Abstract: | Hagfish, the plesiomorphic sister group of all vertebrates, are deep-sea scavengers. The large musculus (m.) longitudinalis linguae (dental muscle) is a specialized element of the feeding apparatus that facilitates the efficient ingestion of food. In this
article, we compare the protein expression in hagfish dental and somatic (the m. parietalis) skeletal muscles via two-dimensional gel electrophoresis and mass spectrometry in order to characterize the former muscle.
Of the 500 proteins screened, 24 were identified with significant differential expression between these muscles. The proteins
that were overexpressed in the dental muscle compared to the somatic muscle were troponin C (TnC), glycogen phosphorylase,
β-enolase, fructose-bisphosphate aldolase A (aldolase A), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). In contrast,
myosin light chain 1 (MLC 1) and creatine kinase (CK) were over-expressed in the somatic muscle relative to the dental muscle.
These results suggest that these two muscles have different energy sources and contractile properties and provide an initial
representative map for comparative studies of muscle-protein expression in low craniates. |
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Keywords: | dental muscle hagfish proteomics two-dimensional electrophoresis white muscle |
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