Determination of sulfinpyrazone and two of its metabolites in human plasma and urine by gas chromatography and selective detection |
| |
Authors: | P. Jakobsen A.Kirstein Pedersen |
| |
Affiliation: | Department of Pharmacology, University of Aarhus, DK-8000 Aarhus C Denmark |
| |
Abstract: | A selective and sensitive gas chromatographic method for simultaneous determination of sulfinpyrazone and two of its metabolites (the para-hydroxylated metabolite and the sulfone metabolite) in biological fluids using alkali flame ionization detection (AFID), electron capture detection (ECD) and mass fragmentographic detection is described. The compounds are extracted from the samples, methylated and separated on 2% OV-17 or 8% OV-225 columns. Phenylbutazone is used as internal standard. Standard curves are linear. The coefficient of variation at 10 μg/ml of sulfinpyrazone in plasma was shown to be 1.8% (AFID), and the detection limits were 0.1 μg/ml (AIFD) and 10 ng/ml (ECD). Mass spectra of the methylated compounds are shown and serum concentration curves after oral administration of 100 mg sulfinpyrazone to two persons are determined together with the excreted amounts of drug and metabolites. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |