High-performance liquid chromatographic analysis of biogenic amines in biological materials as o-phthalaldehyde derivatives |
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| Authors: | Thomas P. Davis Charles W. Gehrke Charles W. Gehrke Thomas D. Cunningham Kenneth C. Kuo Klaus O. Gerhardt Harold D. Johnson Charles H. Williams |
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| Affiliation: | Physiology Area, University of Missouri, Columbia, Mo. 65211 U.S.A.;Department of Biochemistry, Experiment Station Chemical Laboratories, University of Missouri, Columbia, Mo. 65211 U.S.A.;Department of Dairy Husbandry, University of Missouri, Columbia, Mo. 65211 U.S.A.;Sinclair Comparative Medicine Research Farm, University of Missouri, Columbia, Mo. 65211 U.S.A. |
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| Abstract: | A remarkably sensitive, simple and selective reversed-phase high-performance liquid chromatographic (HPLC) method has been developed, allowing, for the first time, the direct measurement of histamine, norepinephrine, octopamine, normetanephrine, dopamine, serotonin and tyramine in a single sample of plasma (2 ml), tissue (0.2 g), or urine. The biogenic amines were modified by pre-column derivatization with o-phthalaldehyde which stabilizes the molecules, aids in extraction, and improves HPLC detection at the nanogram level. To minimize losses during the sampling procedure a careful collection procedure was designed. |
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| Keywords: | To whom correspondence should be addressed at the following address: Room 4 Agriculture Building University of Missouri Columbia Mo. 65211 U.S.A. |
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