Determination of phenylalanine in serum using reversed-phase liquid chromatography and fluorescence detection |
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Authors: | G.J. Schmidt D.C. Olson W. Slavin |
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Affiliation: | The Perkin-Elmer Corporation, Norwalk, Conn. 06856 U.S.A. |
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Abstract: | A liquid chromatography procedure is reported for determining phenylalanine in small volumes of serum. A 10-μl volume of serum was deproteinized with ethanol and an aliquot was derivatized with dansyl chloride reagent. The dansylated phenylalanine and the norleucine internal standard were separated using reversed-phase chromatography and measured with a fluorescence detector. Linearity was excellent over the range 50–800 mg/l. Within-run precision was better than 4%. Total analysis time including chromatography was approximately 40 min. As little as 300 pg of dansylated phenylalanine was detected. |
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