Simple method for the determination of choline and acetylcholine by pyrolysis gas chromatography |
| |
| Authors: | Yuji Maruyama Masaru Kusaka Jun Mori Akiko Horikawa Yoshikazu Hasegawa |
| |
| Institution: | Japan Upjohn Research Laboratories, 168 Oyagi-machi, Takasaki-City, Gunma Prefecture 370 Japan |
| |
| Abstract: | An improved purification procedure is described for the simultaneous assay of endogenous choline and acetylcholine by pyrolysis gas chromatography, particularly for providing a simple and effective method for propionylation of choline in the presence of acetylcholine. The reaction was carried out in acetonitrile solution prepared by dissolving the evaporated residue of the supernatant of brain homogenate. Thus samples for propionylation were prepared without the use of ion-exchange chromatography. |
| |
| Keywords: | To whom correspondence should be addressed |
| 本文献已被 ScienceDirect 等数据库收录！ |
