Role of <Emphasis Type="Italic">Yersinia enterocolitica</Emphasis> heat-stable enterotoxin (Y-STa) on differential regulation of nuclear and cytosolic calcium signaling in rat intestinal epithelial cells |
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Authors: | Subhrajit Saha Pinki Chowdhury Arindam Mazumdar Amit Pal Pradeep Das Manoj K Chakrabarti |
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Institution: | (1) Division of Pathophysiology, National Institute of Cholera and Enteric Diseases, P-33, C.I.T.Road, Scheme-XM, Beliaghata, Calcutta, 700010, India |
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Abstract: | The heat-stable enterotoxin (Y-STa) produced by the pathogenic strains of Yersinia enterocolitica is a causative agent of secretory diarrhea. We have reported earlier that Y-STa-induced inositol trisphosphate-mediated cytosolic
calcium rise occurs in rat intestinal epithelial cells. In the present communication, the involvement of a nuclear calcium
store in the action mechanism of Y-STa in rat intestinal epithelial cells has been shown. Calcium imaging with time series
confocal microscopy shows that Y-STa stimulates both the nuclear and cytosolic calcium levels in rat intestinal epithelial
cells where a rise in nuclear calcium precedes the cytosolic events. Moreover, Y-STa stimulates both cytosolic and nuclear
inositol trisphosphate (IP3) levels in a time-dependent manner. Western blot and immunocytochemical analysis reveal a higher density of IP3 receptor type II in the nuclear membrane compared to the cytosol, which may be the cause of an early rise of the nuclear
calcium level. Therefore, it is suggested that Y-STa regulates the nuclear and cytosolic calcium signals in a distinct temporal
manner in rat intestinal epithelial cells. |
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Keywords: | Calcium Heat-stable enterotoxin Inositol trisphosphate (IP3) receptor Rat intestinal epithelial cells |
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