In vitro reactivation of sarin-inhibited brain acetylcholinesterase from different species by various oximes |
| |
Authors: | K. Kuča J. Cabal |
| |
Affiliation: | Faculty of Military Health Sciences, Department of Toxicology, PO BOX 35/T, 500 01, Hradec Králové, Czech Republic |
| |
Abstract: | In vitro as well as in vivo evaluation of the reactivating efficacy of various oximes against nerve agent-inhibited acetylcholinesterase has been usually done with the help of animal experiments. Nevertheless, previously published data indicate that the reactivation potency of oximes may be different in human and animal species, which may hamper the extrapolation of animal data to human data. Therefore, to better evaluate the efficacy of various oximes (pralidoxime, obidoxime, HI-6, K033) to reactivate brain acetylcholinesterase inhibited by sarin by in vitro methods, human, rat and pig brain acetylcholinesterase were used to calculate kinetic parameters for the reactivation. Our results show differences among the species, depending on the type of oxime, and indicate that data from animal experiments needs to be carefully evaluated before extrapolation to humans. |
| |
Keywords: | Sarin oximes reactivation species differences acetylcholinesterase in vitro |
|
|