Phenotypic plasticity of melanocytes derived from human adult skin |
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| Authors: | D niel L szl Vid cs,Zolt n Ver b,Ren ta Boz ,Lili Borb la Flink,Hilda Poly nka,Istv n Bal zs N meth,Szil rd P liska,Benjamin Tam s Papp,M t Manczinger,R bert G sp r,Seyedmohsen Mirdamadi,Lajos Kem ny,Zsuzsanna Bata-Cs rg&#x |
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| Affiliation: | Dániel László Vidács,Zoltán Veréb,Renáta Bozó,Lili Borbála Flink,Hilda Polyánka,István Balázs Németh,Szilárd Póliska,Benjamin Tamás Papp,Máté Manczinger,Róbert Gáspár,Seyedmohsen Mirdamadi,Lajos Kemény,Zsuzsanna Bata-Csörgő |
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| Abstract: | We previously described a novel in vitro culture technique for dedifferentiated human adult skin melanocytes. Melanocytes cultured in a defined, cholera toxin and PMA free medium became bipolar, unpigmented, and highly proliferative. Furthermore, TRP-1 and c-Kit expression disappeared and EGFR receptor and nestin expression were induced in the cells. Here, we further characterized the phenotype of these dedifferentiated cells and by comparing them to mature pigmented melanocytes we detected crucial steps in their phenotype change. Our data suggest that normal adult melanocytes easily dedifferentiate into pluripotent stem cells given the right environment. This dedifferentiation process described here for normal melanocyte is very similar to what has been described for melanoma cells, indicating that phenotype switching driven by environmental factors is a general characteristic of melanocytes that can occur independent of malignant transformation. |
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| Keywords: | dedifferentiation melanocyte stem cells melanocytes melanoma melanoma stem cells |
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