Screening for acetylcholinesterase inhibitory activity in cyanobacteria of the genus Nostoc |
| |
| Authors: | Petr Zelík Alena Lukešová Ludmila N. Voloshko Dalibor Štys Jiří Kopecký |
| |
| Affiliation: | 1. zelik@fch.vutbr.cz;3. Institute of Soil Biology, Biology Centre of the Academy of Sciences of the Czech Republic, ?eské Budějovice, Czech Republic;4. Research Institute of Biology, SpbGU, St. Petersburg, Russia;5. Institute of Physical Biology, University of South Bohemia, Nové Hrady, Czech Republic;6. Department of Autotrophic Microorganisms, Institute of Microbiology, Academy of Sciences of the Czech Republic, T?eboň, Czech Republic |
| |
| Abstract: | Fifty-four cyanobacterial strains of the genus Nostoc from different habitats were screened for acetylcholinesterase inhibitory activity. Water-methanolic extracts from freeze-dried biomasses were tested for inhibitory activity using Ellman's spectrophotometric method. Acetylcholinesterase inhibitory activity higher than 90% was found in the crude extracts of Nostoc sp. str. Luke?ová 27/97 and Nostoc ellipsosporum Rabenh. str. Luke?ová 51/91. Extracts from Nostoc ellipsosporum str. Luke?ová 52/91 and Nostoc linckia f. muscorum (Ag.) Elenk. str. Gromov, 1988, CALU-980 inhibited AChE activity by 84.9% and 65.3% respectively. Moderate AChE inhibitory activity (29.1–37.5%) was found in extracts of Nostoc linckia Roth. str. Gromov, 1962/10, CALU-129, Nostoc muscorum Ag. str. Luke?ová 127/97, Nostoc sp. str. Lhotsky, CALU-327 and Nostoc sp. str. Gromov, CALU-998. Extracts from another seven strains showed weak anti-AChE activities.The active component responsible for acetylcholinesterase inhibition was identified in a crude extract of Nostoc sp. str. Luke?ová 27/97 using HPLC and found to occur in one single peak. |
| |
| Keywords: | Acetylcholinesterase Alzheimer's disease bioactivity inhibitors microalgae Nostoc |
|
|
