Monitoring the Inhibitive Effect of the Static Magnetic Field on the Activity of Lysozyme with Acoustic Wave Impedance Analysis Technique

The inhibitive effect of static magnetic field on the activity of lysozyme was studied using acoustic wave impedance analysis technique. Equivalent circuit parameters of piezoelectric quartz crystal (PQC) were obtained and discussed. The results showed that the activity of lysozyme was inhibited due to the effect of static magnetic field and the inhibitive effect becomes greater with an increase in magnetization time or magnetic field intensity. According to the response characteristics of motional resistance change (ΔR₁), which is related to the change in the bacterial number, a quantitative response model reflecting the activity of lysozyme was theoretically derived. By fitting ΔR₁ versus time curves under a specific magnetic field intensity but different magnetic time to the model, the relationship between K₁ reflecting the activity of lysozyme and magnetic time t_m was established. Based on the relationship, a new impedance response model that indicates the inhibitive influence of the magnetization time on the activity of lysozyme was derived as follows: ΔR₁=R₀{{K₄{expK₀?exp(?0.26t_m)]t?1}+1}^1/2?1}. Similarly, another response model that indicates the effect of magnetic field intensity was derived as follows: ΔR₁=R₀{{K₄{exp(K₀?exp(?5.17B)t)?1}+1}^1/2?1}.