Purification and characterisation of an inhibitor of a cathepsin B-like proteinase from sunflower seed |
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Authors: | Xin-Chang Zhang Hong-Lian Shao Jin-Xing Wang Xiao-Fan Zhao |
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Affiliation: | School of Life Sciences, Shandong University, Jinan, 250100, China |
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Abstract: | Cathepsin B is a vitally important enzyme in various physiological processes and in tumor invasion and metastasis. A cathepsin B inhibitor, HCB-SunI, was identified and purified from sunflower seeds, Helianthus annuus, using ammonium sulfate precipitation and two steps of conventional chromatography. The molecular mass of HCB-SunI was estimated to be 12 kDa by SDS-PAGE and 12.32 kDa by MALDI TOF MS. Its N-terminal amino acid sequence was determined to be: PYGGGGTESG. HCB-SunI not only inhibited Helicoverpa cathepsin B (HCB) but also decreased the growth of HeLa and glioma cells by 7 ~ 27% and 6 ~ 22%, respectively, when the cells were grown in a final concentration of 0.002 ~ 0.008 μM inhibitor. |
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Keywords: | Helianthus annuus Cathepsin B Inhibitor Purification Tumor cells effect sunflower seed |
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