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Tyrosinase inhibitory effect and inhibitory mechanism of tiliroside from raspberry |
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| Authors: | Yan-hua Lu Juan Chen Dong-zhi Wei Zheng-tao Wang Xin-yi Tao |
| Affiliation: | 1. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR Chinaluyanhua@ecust.edu.cn;3. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China;4. Institute of Chinese Materia Medica, Shanghai University of TCM, Shanghai, 201203, PR China |
| Abstract: | Tiliroside was found to inhibit both monophenolase and diphenolase activity of mushroom tyrosinase. The lag time of tyrosine oxidation catalyzed by mushroom tyrosinase was obviously lengthened; 0.337?mM of tiliroside resulted in the lag time extension from 46.7?s to 435.1?s. A kinetic analysis shown that tiliroside was a competitive inhibitor for monophenolase and diphenolase with Ki values of 0.052?mM and 0.26?mM, respectively. Furthermore, tiliroside showed 34.5% (p? |
| Keywords: | Raspberry tiliroside Rubus chingii hu mushroom tyrosinase inhibition mechanism B16 mouse melanoma cells melanin |