Tyrosinase inhibitory effect and inhibitory mechanism of tiliroside from raspberry |
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| Authors: | Yan-hua Lu Juan Chen Dong-zhi Wei Zheng-tao Wang Xin-yi Tao |
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| Institution: | 1. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR Chinaluyanhua@ecust.edu.cn;3. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China;4. Institute of Chinese Materia Medica, Shanghai University of TCM, Shanghai, 201203, PR China |
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| Abstract: | Tiliroside was found to inhibit both monophenolase and diphenolase activity of mushroom tyrosinase. The lag time of tyrosine oxidation catalyzed by mushroom tyrosinase was obviously lengthened; 0.337?mM of tiliroside resulted in the lag time extension from 46.7?s to 435.1?s. A kinetic analysis shown that tiliroside was a competitive inhibitor for monophenolase and diphenolase with Ki values of 0.052?mM and 0.26?mM, respectively. Furthermore, tiliroside showed 34.5% (p?<?0.05) inhibition of intracellular tyrosinase activity and 54.1% (p?<?0.05) inhibition of melanin production with low cytotoxicity on B16 mouse melanoma cells at 0.168?mM. In contrast, arbutin displayed 9.1% inhibition of cellular tyrosinase activity and 29.5% inhibition of melanin production at the same concentration. These results suggested that tiliroside was a potent tyrosinase inhibitor and might be used as a skin-whitening agent and pigmentation medicine. |
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| Keywords: | Raspberry tiliroside Rubus chingii hu mushroom tyrosinase inhibition mechanism B16 mouse melanoma cells melanin |
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